TS Spurrs - FFPE block - 1mm3 cube cut out - tissue (TM - 013)
sandra.crameri
Published: 2023-05-09 DOI: 10.17504/protocols.io.kxygx968wg8j/v1
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Abstract
Processing unstained parraffin block that has had a 1mm3 piece disected out of the face to Spurr's resin block for EM.
Steps
HEADER
1.
SAN:
SPEC No:
OPERATOR & STEPS:
OPERATOR & STEPS:
Dewax FFPE sections
2.
0h 5m 0s at 60°C
3.
0h 5m 0s at Room temperature
4.
0h 5m 0s at Room temperature
Rehydrate
5.
100% volume 0h 10m 0s
6.
95% volume 0h 10m 0s
7.
70% volume 0h 10m 0s
Conventional - with reduced times for 5um thick FFPE section
8.
2.5%% volume 0.1Molarity (M) Sorenson's phosphate buffer 07.2 300 mosmol/kg0h 20m 0s.
9.
Wash buffer 0h 5m 0s
10.
1Mass / % volume 1h 0m 0s
11.
70% volume 0h 10m 0s
12.
95% volume 0h 10m 0s
13.
100% volume 0h 10m 0s
14.
100% volume 0h 10m 0s
15.
100% volume Spurrs:100% volume 0h 30m 0s
16.
100% volume Spurrs1h 0m 0s
17.
100% volume Spurrs1h 0m 0s
18.
Oven polymerise 65°C
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