NCEM Drop - Paramyxo confirmation, generation of RNP (TM-014)
sandra.crameri
Published: 2023-05-13 DOI: 10.17504/protocols.io.x54v9dq8pg3e/v1
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Abstract
Generate RNP to confirm Paramyxoviurs
Steps
HEADER
1.
SAN:
SPEC No:
OPERATOR:
Generation of RNP
2.
Prepare 0.5Mass / % volume
3.
Mix 1:1 with the sample for 0h 5m 0s
Conventional
4.
Adsorb 10µL sample to grid 0h 10m 0s , inspect to ensure sample does not dry out.
5.
Drain excess sample from grid using filter paper, leave wet.
6.
Stain 0h 1m 0s
7.
Drain & dry using filter paper
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